About The GENSAT project at Rockefeller University

The GENSAT project aims to map the expression of genes in the central nervous system of the mouse, using both in situ hybridization and transgenic mouse techniques. It is a collection of pictorial gene expression maps of the brain and spinal cord of the mouse. Using EGFP BAC-transgenics, this project provides the scientific community with tools to catalog, map, and electrophysiologically record from individual cells. Our application of Cre recombinase technologies allows for cell-specific gene manipulation. The transgenic mice created by this project are available to the scientific community.

GENSAT Community
GENSAT Protocols
  • Transgenic Core Protocol
  • Histology Core Protocol
  • BAC Core Protocol
    Using our BAC engineering system, we introduce an EGFP, tdTomato or Cre recombinase gene upstream of the ATG start codon of the gene of interest on a selected BAC clone. The inserted gene has its own ATG start codon and polyadenylation signal. This modification results in the expression of EGFP, tdTomato or Cre recombinase that is under the control of the promoter and regulatory elements of the gene of interest that are present on the BAC clone.

GENSAT Data Mining
Contact, tutorial, troubleshooting
Contents of this work may be downloaded, copied, and cited. The data are provided without warranty as to the accuracy, completeness or fitness for any specific use. The GENSAT database and its contents may not be redistributed or sold, in whole or in part, without specific written permission. The recommended citation for the resource is: "The Gene Expression Nervous System Atlas (GENSAT) Project, NINDS Contracts N01NS02331 & HHSN271200723701C to The Rockefeller University (New York, NY)."

Thanks to Mona and Tom Nemoda for creating the GENSAT logo.

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