NINDS GENSAT BAC Transgenics Project
The Rockefeller University

 
The Gensat database contains a gene expression atlas of the central nervous system of the mouse based on bacterial artificial chromosomes (BACs). In each of the BAC transgenic vectors, endogenous protein coding sequences have been replaced by sequences encoding the EGFP reporter gene. As in any gene replacement experiment, the stability of the reporter gene can vary somewhat from the endogenous gene. Thus these results measure the relative rates of transcription for each gene; they are not a direct measure of mRNA accumulation or of protein abundance for the endogenous gene products. Furthermore, the enhanced sensitivity of reporter gene assays, particularly in BAC lines carrying multiple copies of the BAC transgene may allow detection of sites of expression that are not evident in situ hybridization experiments. This database contains histological data from given BAC transgenic mouse lines at three developmental stages embryonic day 15.5 (E15.5), postnatal day 7 (P7) and adult; in all cases the data represent results of multiple transgenic lines. EGFP is visualized by staining with an anti-EGFP antibody using the DAB method, or by confocal microscopy of unstained tissue sections. Protocols for the modification of BACs, BAC transgenesis production and histology are provided.

Contents of this work may be downloaded, copied, and cited.
The data are provided without warranty as to the accuracy, completeness or fitness for any specific use.
The GENSAT database and its contents may not be redistributed or sold, in whole or in part, without specific written permission.
The recommended citation for the resource is:
The Gene Expression Nervous System Atlas (GENSAT) Project, NINDS Contract # N01NS02331 to The Rockefeller University (New York, NY).


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