Section Gene Symbol Age Orientation Objective Veracity Veracity Support 07 potassium inwardly-rectifying channel, subfamily J, member 5 Kcnj5 adult Sagittal 10X Confirmed Multiple Lines Match Supported by Literature

BX BAC Address marker BX99 RP23-111B14 EGFP

Other cross-sections of this brain: 01 02 03 04 05 06 07 08 09 10 (thumbnails not to scale)

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Add this image to your album Download this image at the scale shown below. Founder Line: BZ49 See all MMRRC strains for : Kcnj5 Get this mouse from MMRRC: STOCK Tg(Kcnj5-EGFP)BZ49Gsat/Mmucd: Details, Order Get this BAC Clone from CHORI: BX99 UCSC Genome Browser for this BAC Address: RP23-111B14

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Comment on this Gene: 6 BAC lines are identical at P7 (three of them identical at E15-5 and adult too). Our BAC data matches the literature in general. In cortex, layer VI neurons express EGFP transgene at P7, then the expression declines to undetectable level in adult. This pattern is consistent with the published data. But there are a few disagreements: 1. Our BAC also labels layer1 of the cerebral cortex, which is not documented in the literature. 2. Strong expression in claustrum is not seen in our data. 3. At E15-5, our BAC stains a few more structures in brain other than the ventromedial hypothalamic nucleus.

Gene Expression Annotations for this image

structure	Expression Level	Expression Pattern	Cell types	Subtypes
Olfactory Bulb	moderate to strong signal	region-specific	neuron	periglomerular cell of AOB other
Cerebral cortex	weak signal	region-specific	neuron	fibers
Hippocampus	moderate to strong signal	region-specific	neuron	interneuron
Caudate Putamen	undetectable	ND
Ventral striatum	moderate to strong signal	scattered		fibers
Basal Forebrain	moderate to strong signal	region-specific and scattered	neuron
Thalamus	moderate to strong signal	region-specific	neuron
Hypothalamus	moderate to strong signal	region-specific and scattered	neuron
Midbrain	moderate to strong signal	region-specific and scattered	neuron	fibers
Substantia Nigra	moderate to strong signal	region-specific	neuron
Pons	weak signal	region-specific	neuron
Medulla	moderate to strong signal	scattered	neuron
Cerebellum	moderate to strong signal	region-specific	neuron	interneuron
Optic tract	moderate to strong signal	ND

Other image sets for Kcnj5

age	orientation	Acquisition technique	Founder Line	Batch
adult	Horizontal	brightfield	BZ49	3 images
adult	Horizontal	confocal	BZ49	1 images
adult	Sagittal	brightfield	BZ49	10 images
adult	Sagittal	confocal	BZ49	8 images
P7	Horizontal	brightfield	BZ49	3 images
P7	Sagittal	brightfield	BZ49	11 images
P7	Sagittal	confocal	AN101	7 images
E15.5	Horizontal	brightfield	BZ73	3 images
E15.5	Sagittal	brightfield	BZ73	10 images
E15.5	Sagittal	confocal	BZ49	4 images
E15.5	Sagittal	confocal	AN101	1 images

BGEM Probe	Related ISH images
Kcnj5	308

Supporting literature:

PubMed ID
10908597
8642402
9044369
9532576

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Veracity	definition
Confirmed	Confirmed refers to genes in which multiple lines yield matching datasets that agree with the available literature, or in which a single line has been produced that matches the available literature. In those cases where there a discrepancy between the literature and the reporter gene data (missing or additional sites of expression), this is noted in the database.
To Be Confirmed	To be confirmed refers to genes in which multiple lines produce matching datasets, but for which no independent expression data is available.
Anomalous	Anomalous refers to data collected from BAC reporter constructs producing different expression patterns in different lines (i.e. position effects).

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