Structure Gene Symbol Age Orientation Objective Veracity Veracity Support Cerebellar Nuclei neurofilament, heavy polypeptide Nefh adult Sagittal 10X Confirmed Matches In Situ Data Supported by Literature

BX BAC Address marker BX1256 RP23-64E17 EGFP

Other structures in this brain: CEN CER CER CER1 CEX MED MED1 MID MID1 MID2 PON PON1 THA

Add this image to your album Download this image at the scale shown below. Founder Line: GY27 See all MMRRC strains for : Nefh Get this mouse from MMRRC: STOCK Tg(Nefh-EGFP)GY27Gsat/Mmucd: Details, Order Get this BAC Clone from CHORI: BX1256 UCSC Genome Browser for this BAC Address: RP23-64E17

Read gene expression annotations for this image View other image sets for Nefh

approximately section 06/07

Comment on this Gene: Limited expression literature. The BAC data is consistent with the cited paper and the adult in situ from Allen database. In hippocampus, the literature reports that Nefh mRNA is localized in the hippocampus. Only scattered cells are labeled in the BAC data, which is supported by Allen in situ data. Our BAC also expresses in a few brain areas, such as cerebellum, midbrain and medulla, that are confirmed by the Allen data. In cerebellum, the expression in Purkinje neurons is mosaic in the BAC mice.

Gene Expression Annotations for this image

structure	Expression Level	Expression Pattern	Cell types	Subtypes
Cerebellar Nuclei	moderate to strong signal	region-specific	neuron	fibers

Other image sets for Nefh

age	orientation	Acquisition technique	Founder Line	Batch
adult	Horizontal	brightfield	GY27	3 images
adult	Horizontal	confocal	GY27	1 images
adult	Sagittal	brightfield	GY27	10 images
adult	Sagittal	confocal	GY27	13 images
P7	Horizontal	brightfield	GY27	3 images
P7	Horizontal	confocal	GY27	2 images
P7	Sagittal	brightfield	GY27	11 images
P7	Sagittal	confocal	GY27	13 images
E15.5	Horizontal	brightfield	GY27	3 images
E15.5	Horizontal	confocal	GY27	1 images
E15.5	Sagittal	brightfield	GY27	10 images
E15.5	Sagittal	confocal	GY27	1 images

BGEM Probe	Related ISH images
Nefh	2891

Supporting literature:

PubMed ID
2070748

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Veracity	definition
Confirmed	Confirmed refers to genes in which multiple lines yield matching datasets that agree with the available literature, or in which a single line has been produced that matches the available literature. In those cases where there a discrepancy between the literature and the reporter gene data (missing or additional sites of expression), this is noted in the database.
To Be Confirmed	To be confirmed refers to genes in which multiple lines produce matching datasets, but for which no independent expression data is available.
Anomalous	Anomalous refers to data collected from BAC reporter constructs producing different expression patterns in different lines (i.e. position effects).

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