Section Gene Symbol Age Orientation Objective Veracity Veracity Support 11 asp (abnormal spindle)-like, microcephaly associated (Drosophila) Aspm P7 Sagittal 10X Confirmed Multiple Lines Match Matches In Situ Data

BX BAC Address marker BX1643 RP24-267F8 EGFP

Other cross-sections of this brain: 01 02 03 04 05 06 07 08 09 10 11 (thumbnails not to scale)

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Add this image to your album Download this image at the scale shown below. Founder Line: IH113 See all MMRRC strains for : Aspm Get this mouse from MMRRC: STOCK Tg(Aspm-EGFP)IH113Gsat/Mmucd: Details, Order Get this BAC Clone from CHORI: BX1643 UCSC Genome Browser for this BAC Address: RP24-267F8

Read gene expression annotations for this image View other image sets for Aspm

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Comment on this Gene: Mutation in Aspm gene is associated with primary microcephaly. Aspm protein is localized in mitotic spindle in progenitor cells. The BAC data is reproducible and consistent with BGEM in situ with highest levels of expression in cerebellar EGL, RMS and cells in subventricular zone at P7. The cerebellar IGL is also stained due to the stability of EGFP protein. At adult, EGFP transgene expression remains in RMS and SVZ. At E15-5, the BAC expresses in cells in the ventricular zone. However, the postmitotic cells are labeled due to the stability of EGFP protein.

Gene Expression Annotations for this image

structure	Expression Level	Expression Pattern	Cell types	Subtypes
Olfactory Bulb	moderate to strong signal	region-specific and scattered	neuron
Cerebral cortex	moderate to strong signal	region-specific and scattered	neuron glial cell
Septum	moderate to strong signal	scattered	glial cell
Thalamus	moderate to strong signal	scattered	glial cell
Hypothalamus	moderate to strong signal	region-specific and scattered	glial cell
Midbrain	moderate to strong signal	region-specific and scattered	neuron glial cell
Pons	moderate to strong signal	region-specific and scattered	neuron glial cell
Medulla	moderate to strong signal	region-specific and scattered	neuron glial cell
Cerebellum	moderate to strong signal	region-specific	neuron	granule neuron of cerebellum

Other image sets for Aspm

age	orientation	Acquisition technique	Founder Line	Batch
adult	Horizontal	brightfield	IH113	3 images
adult	Sagittal	brightfield	IH113	10 images
P7	Horizontal	brightfield	IH113	3 images
P7	Sagittal	brightfield	IH113	11 images
P7	Sagittal	confocal	IH113	3 images
E15.5	Horizontal	brightfield	IH113	3 images
E15.5	Horizontal	confocal	IH113	1 images
E15.5	Sagittal	brightfield	IH113	10 images
E15.5	Sagittal	confocal	IH113	4 images
E15.5	Sagittal	confocal	IH113	4 images

BGEM Probe	Related ISH images
Aspm	2336

Supporting literature:

PubMed ID
12355089
16798874
12351193
15972725

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Veracity	definition
Confirmed	Confirmed refers to genes in which multiple lines yield matching datasets that agree with the available literature, or in which a single line has been produced that matches the available literature. In those cases where there a discrepancy between the literature and the reporter gene data (missing or additional sites of expression), this is noted in the database.
To Be Confirmed	To be confirmed refers to genes in which multiple lines produce matching datasets, but for which no independent expression data is available.
Anomalous	Anomalous refers to data collected from BAC reporter constructs producing different expression patterns in different lines (i.e. position effects).

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