Section Gene Symbol Age Orientation Objective Veracity Veracity Support 03 neurofilament, light polypeptide Nefl E15.5 Sagittal 10X Confirmed Multiple Lines Match Matches In Situ Data

BX BAC Address marker BX1261 RP24-327L23 EGFP

Other cross-sections of this brain: 01 02 03 04 05 06 07 08 09 10 (thumbnails not to scale)

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Add this image to your album Download this image at the scale shown below. Founder Line: GZ84 See all MMRRC strains for : Nefl Get this mouse from MMRRC: STOCK Tg(Nefl-EGFP)GZ84Gsat/Mmucd: Details, Order Get this BAC Clone from CHORI: BX1261 UCSC Genome Browser for this BAC Address: RP24-327L23

Read gene expression annotations for this image View other image sets for Nefl

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Comment on this Gene: No detailed expression literature found. Two BAC lines have almost identical expression at P7 except in the hippocampus. The BAC data is correct but incomplete. In P7 cortex, cells are very weakly labeled in the right layers (only clearly visible for some of the sections, such as 05 and 06 sections). But the cortical expression is evident in adult BAC mice and matches the in situ data. In hippocampus, the in situ data shows transient presence of Nefl mRNA in the subiculum at P7. No expression in the subiculum is seen in P7 BAC mice. In addition, only a few cells are faintly stained in the CA region in the P7 BAC data. The BAC exhibits an interesting, column-like expression pattern in the superior colliculus, which is not clear by the in situ.

Gene Expression Annotations for this image

structure	Expression Level	Expression Pattern	Cell types	Subtypes
Cerebral cortex	weak signal	region-specific
Hippocampus	undetectable	ND
Caudate Putamen	moderate to strong signal	region-specific
Thalamus	moderate to strong signal	region-specific
Midbrain	moderate to strong signal	region-specific
Pons+Medulla	moderate to strong signal	region-specific
Cerebellum	moderate to strong signal	region-specific

Other image sets for Nefl

age	orientation	Acquisition technique	Founder Line	Batch
adult	Horizontal	brightfield	GZ84	3 images
adult	Horizontal	confocal	GZ84	1 images
adult	Horizontal	confocal	GZ84	1 images
adult	Horizontal	confocal	GZ84	1 images
adult	Sagittal	brightfield	GZ84	10 images
adult	Sagittal	confocal	GZ84	13 images
P7	Horizontal	brightfield	GZ84	3 images
P7	Horizontal	confocal	GZ84	2 images
P7	Sagittal	brightfield	GZ84	11 images
P7	Sagittal	confocal	GZ84	14 images
E15.5	Horizontal	brightfield	GZ84	3 images
E15.5	Sagittal	brightfield	GZ84	10 images
E15.5	Sagittal	confocal	GZ84	5 images

BGEM Probe	Related ISH images
Nefl	1737

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Veracity	definition
Confirmed	Confirmed refers to genes in which multiple lines yield matching datasets that agree with the available literature, or in which a single line has been produced that matches the available literature. In those cases where there a discrepancy between the literature and the reporter gene data (missing or additional sites of expression), this is noted in the database.
To Be Confirmed	To be confirmed refers to genes in which multiple lines produce matching datasets, but for which no independent expression data is available.
Anomalous	Anomalous refers to data collected from BAC reporter constructs producing different expression patterns in different lines (i.e. position effects).

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