Section Gene Symbol Age Orientation Objective Veracity Veracity Support 11 galanin Gal P7 Sagittal 10X Confirmed Matches In Situ Data Supported by Literature

BX BAC Address marker BX123 RP23-15L21 EGFP

Other cross-sections of this brain: 01 02 03 04 05 06 07 08 09 10 11 (thumbnails not to scale)

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Add this image to your album Download this image at the scale shown below. Founder Line: HX109 See all MMRRC strains for : Gal Get this mouse from MMRRC: STOCK Tg(Gal-EGFP)HX109Gsat/Mmucd: Details, Order Get this BAC Clone from CHORI: BX123 UCSC Genome Browser for this BAC Address: RP23-15L21

Read gene expression annotations for this image View other image sets for Gal

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Comment on this Gene: The BAC data is consistent with the literature and the in situ data from Allen database. Correct expression sites include hypothalamus, basal forebrain, amygdala, locus coeruleus, raphe nuclei and the nucleus tractus solitarii. There are papers reporting Gal mRNA is expressed in cerebellar Purkinje cells. The BAC data shows EGFP transgene expression predominantly in cerebellar granule neurons. The BAC also detects expression in MOB, dentate gyrus (mainly at P7) and scattered cells in cortex at P7. BGEM in situ failed to detect any specific hybridization signals. At E15-5, the expression in trigeminal ganglia and dorsal spinal cord is confirmed by the E14-5 in situ data from Genepaint.

Gene Expression Annotations for this image

structure	Expression Level	Expression Pattern	Cell types	Subtypes
Olfactory Bulb	moderate to strong signal	region-specific	neuron	fibers
Cerebral cortex	weak signal	scattered	neuron	fibers
Septum	moderate to strong signal	region-specific	neuron	fibers
Thalamus	moderate to strong signal	region-specific	neuron	fibers
Hypothalamus	moderate to strong signal	region-specific	neuron	fibers
Midbrain	moderate to strong signal	region-specific	neuron	fibers
Pons	moderate to strong signal	region-specific	neuron	fibers
Medulla	moderate to strong signal	region-specific	neuron	fibers
Cerebellum	moderate to strong signal	region-specific	neuron	granule neuron of cerebellum
Corpus callosum	moderate to strong signal	region-specific

Other image sets for Gal

age	orientation	Acquisition technique	Founder Line	Batch
adult	Coronal	brightfield	KI87	66 images
adult	Horizontal	brightfield	HX109	3 images
adult	Sagittal	brightfield	HX109	10 images
adult	Sagittal	confocal	HX109	5 images
P7	Horizontal	brightfield	HX109	3 images
P7	Sagittal	brightfield	HX109	11 images
P7	Sagittal	confocal	HX109	3 images
P7	Sagittal	confocal	HX109	4 images
E15.5	Horizontal	brightfield	HX109	3 images
E15.5	Horizontal	confocal	HX109	2 images
E15.5	Horizontal	confocal	HX109	2 images
E15.5	Sagittal	brightfield	HX109	10 images
E15.5	Sagittal	confocal	HX109	5 images

BGEM Probe	Related ISH images
Gal	216

Supporting literature:

PubMed ID
1705056
2457856
2402333
7860799
7534886
8550881
9174078

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Veracity	definition
Confirmed	Confirmed refers to genes in which multiple lines yield matching datasets that agree with the available literature, or in which a single line has been produced that matches the available literature. In those cases where there a discrepancy between the literature and the reporter gene data (missing or additional sites of expression), this is noted in the database.
To Be Confirmed	To be confirmed refers to genes in which multiple lines produce matching datasets, but for which no independent expression data is available.
Anomalous	Anomalous refers to data collected from BAC reporter constructs producing different expression patterns in different lines (i.e. position effects).

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