Section Gene Symbol Age Orientation Objective Veracity Veracity Support 07 cholinergic receptor, muscarinic 4 Chrm4 adult Sagittal 10X Confirmed Matches In Situ Data Supported by Literature

BX BAC Address marker BX74 RP23-138P5 EGFP

Other cross-sections of this brain: 01 02 03 04 05 06 07 08 09 10 (thumbnails not to scale)

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This image is in your album. Download this image at the scale shown below. Founder Line: Y86 See all MMRRC strains for : Chrm4 Get this mouse from MMRRC: STOCK Tg(Chrm4-EGFP)Y86Gsat/Mmnc: Details, Order Get this BAC Clone from CHORI: BX74 UCSC Genome Browser for this BAC Address: RP23-138P5

Read gene expression annotations for this image View other image sets for Chrm4

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Comment on this Gene: Our BAC data is in general consistent with the literature and BGEM in situ data, and provides new data on patterns of expression in medulla, a region lacking expression information. Weak expression in hippocampus is seen in BAC mice, which is in contrast to the literature and the in situ showing stronger expression of Chrm4 in that area. The in situ also reveals high levels of Chrm4 mRNA in a specific nucleus in amygdala, which is relatively weak in the BAC data.

Gene Expression Annotations for this image

structure	Expression Level	Expression Pattern	Cell types	Subtypes
Olfactory Bulb	moderate to strong signal	region-specific	neuron	granule cell of MOB periglomerular cell of MOB
Cerebral cortex	moderate to strong signal	region-specific	neuron	pyramidal cell
Hippocampus	undetectable	ND
Caudate Putamen	moderate to strong signal	widely expressed	neuron
Ventral striatum	moderate to strong signal	widely expressed	neuron
Basal Forebrain	moderate to strong signal	region-specific	neuron
Thalamus	moderate to strong signal	region-specific		fibers
Hypothalamus	moderate to strong signal	region-specific		fibers
Midbrain	weak signal	region-specific		fibers
Pons	moderate to strong signal	scattered	neuron	fibers
Medulla	weak signal	region-specific	neuron

Other image sets for Chrm4

age	orientation	Acquisition technique	Founder Line	Batch
adult	Horizontal	brightfield	Y86	3 images
adult	Sagittal	brightfield	Y86	10 images
adult	Sagittal	confocal	Y86	3 images
P7	Horizontal	brightfield	Y86	3 images
P7	Sagittal	brightfield	Y86	11 images
P7	Sagittal	confocal	Y86	3 images
E15.5	Horizontal	brightfield	Y86	3 images
E15.5	Sagittal	brightfield	Y86	10 images
E10.5	Sagittal	brightfield	Y86	7 images

BGEM Probe	Related ISH images
Chrm4	909

Supporting literature:

PubMed ID
3199198
2052149
10575021

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Veracity	definition
Confirmed	Confirmed refers to genes in which multiple lines yield matching datasets that agree with the available literature, or in which a single line has been produced that matches the available literature. In those cases where there a discrepancy between the literature and the reporter gene data (missing or additional sites of expression), this is noted in the database.
To Be Confirmed	To be confirmed refers to genes in which multiple lines produce matching datasets, but for which no independent expression data is available.
Anomalous	Anomalous refers to data collected from BAC reporter constructs producing different expression patterns in different lines (i.e. position effects).

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