Section Gene Symbol Age Orientation Objective Veracity Veracity Support 07 nitric oxide synthase 1, neuronal Nos1 adult Sagittal 10X Confirmed Multiple Lines Match Matches In Situ Data Supported by Literature

BX BAC Address marker BX628 RP24-164C18 EGFP

Other cross-sections of this brain: 01 02 03 04 05 06 07 08 09 10 (thumbnails not to scale)

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This image is in your album. Download this image at the scale shown below. Founder Line: DP185 See all MMRRC strains for : Nos1 Get this mouse from MMRRC: STOCK Tg(Nos1-EGFP)DP185Gsat/Mmucd: Details, Order Get this BAC Clone from CHORI: BX628 UCSC Genome Browser for this BAC Address: RP24-164C18

Read gene expression annotations for this image View other image sets for Nos1

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Comment on this Gene: The gene was targeted three times with three different BACs in hope to obtain a line with correct expression in interneurons in the cerebral cortex and striatum. However, of all the lines generated, we are unable to find a line that expresses in interneurons in these two brain areas. Instead, the BAC is reproducibly expressed in pyramidal neurons in the cortex in different BAC modifications. Other expression sites observed in BAC data appears to be consistent with the literature and BGEM in situ data in general.

Gene Expression Annotations for this image

structure	Expression Level	Expression Pattern	Cell types	Subtypes
Olfactory Bulb	moderate to strong signal	region-specific and scattered	neuron	fibers
Cerebral cortex	moderate to strong signal	region-specific and scattered	neuron
Piriform cortex	moderate to strong signal	region-specific	neuron	fibers
Hippocampus	moderate to strong signal	region-specific	neuron	fibers
Caudate Putamen	moderate to strong signal	widely expressed	not clear
Ventral striatum	moderate to strong signal	region-specific and scattered	neuron
Basal Forebrain	moderate to strong signal	region-specific	neuron
Thalamus	undetectable	ND
Hypothalamus	moderate to strong signal	widely expressed	neuron	fibers
Midbrain	moderate to strong signal	region-specific and scattered	neuron vascular cell
Pons	moderate to strong signal	region-specific and scattered	neuron vascular cell
Medulla	moderate to strong signal	region-specific and scattered	neuron vascular cell
Cerebellum	moderate to strong signal	region-specific	neuron

Other image sets for Nos1

age	orientation	Acquisition technique	Founder Line	Batch
adult	Horizontal	brightfield	DP185	3 images
adult	Horizontal	confocal	DP185	1 images
adult	Sagittal	brightfield	DP185	10 images
adult	Sagittal	confocal	DP185	4 images
adult	Sagittal	confocal	DP185	5 images
adult	Sagittal	confocal	DP185	6 images
P7	Horizontal	brightfield	DP185	3 images
P7	Sagittal	brightfield	DP185	11 images
P7	Sagittal	confocal	DP185	6 images
E15.5	Horizontal	brightfield	DP185	3 images
E15.5	Sagittal	brightfield	DP185	10 images

BGEM Probe	Related ISH images
Nos1	428

Supporting literature:

PubMed ID
1712077
1718335
7529143

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Veracity	definition
Confirmed	Confirmed refers to genes in which multiple lines yield matching datasets that agree with the available literature, or in which a single line has been produced that matches the available literature. In those cases where there a discrepancy between the literature and the reporter gene data (missing or additional sites of expression), this is noted in the database.
To Be Confirmed	To be confirmed refers to genes in which multiple lines produce matching datasets, but for which no independent expression data is available.
Anomalous	Anomalous refers to data collected from BAC reporter constructs producing different expression patterns in different lines (i.e. position effects).

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